Gene detection of severe acute respiratory syndrome-related coronavirus / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 212-214, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-242198
ABSTRACT
<p><b>OBJECTIVE</b>To develop a newly real-time RT-polymerase chain reaction assay for severe acute respiratory syndrome (SARS) related coronavirus in human whole blood.</p><p><b>METHODS</b>A pair of primers and a probe (molecular beacon) had been designed that were specific for the recognition of a highly conservative region between 15 301 and 15 480 of the SARS-related coronavirus polymerase gene sequences obtained from GenBank (G130027616).</p><p><b>RESULTS</b>In the real-time RT-PCR assay, the extent of SARS related coronavirus amplification was measured in terms of the increase in fluorescence during the amplification process. The 145 bp fragment of PCR product was further confirmed by conventional PCR assay and proved by DNA sequencing to be identical to the target sequence to which the probe was hybridized.</p><p><b>CONCLUSION</b>This assay has a broad application for clinical diagnosis and surveillance investigation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Síndrome Respiratorio Agudo Grave
/
Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo
/
Diagnóstico
/
Genética
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Pathology
Año:
2003
Tipo del documento:
Artículo
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