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High-level production of glucose oxidase by recombinant Pichia pastoris using a combined strategy / 生物工程学报
Chinese Journal of Biotechnology ; (12): 986-990, 2016.
Artículo en Chino | WPRIM | ID: wpr-242281
ABSTRACT
To enhance the production of glucose oxidase by recombinant Pichia pastoris, two strategies were developed, which were namely co-feeding of methanol and sorbitol and co-expressing of the protein disulfide isomerase (PDI) and Vitreoscialla hemoglobin (VHb). The volumetric activity reached 456 U/mL by using the strain X33/pPIC9k-GOD, in 5 liter fermentator, with the co-feeding of methanol and sorbitol, it was 0.2 fold higher than that only feeding by methanol. The improved strain was obtained by co-expressing PDI-VHb with GOD. While fermented in a 5 liter fermentator by feeding methanol and sorbitol, the activity of the improved strain reached 716 U/mL with a yield of 7 400 mg/L total soluble protein concentration. These results indicated that heterologous protein expression level can be enhanced by optimizing fermentation condition and co-expression molecular chaperon in Pichia pastoris.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Pichia / Sorbitol / Proteínas Recombinantes / Reactores Biológicos / Metanol / Fermentación / Glucosa Oxidasa / Metabolismo Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2016 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Pichia / Sorbitol / Proteínas Recombinantes / Reactores Biológicos / Metanol / Fermentación / Glucosa Oxidasa / Metabolismo Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2016 Tipo del documento: Artículo