Seasonal pattern and phylogenetic analysis with human isolates of genotype-IV hepatitis E virus in swine herds, eastern China / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 504-508, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-242626
ABSTRACT
<p><b>OBJECTIVE</b>To determine the seasonal prevalence of genotype-IV hepatitis E virus (HEV) in swine herds in Eastern China and explore the phylogenetic relationship between swine HEV and human HEV in the situation that zoonotic features of HEV had been confirmed.</p><p><b>METHODS</b>From September 2007 to June 2008, a total of 1200 swine bile specimens were collected from three slaughter houses located in Zhejiang, Anhui and Jiangsu, the Eastern China, and detected for HEV RNA by using nested RT-PCR. The positive PCR products were sequenced. Then the swine HEV were phylogenetically determined with human HEV isolated in Eastern China.</p><p><b>RESULTS</b>The positive rate for HEV RNA in swine herds was 4.5% totally. Significant differences of HEV detection were not observed among seasonal pattern (Sep - Oct 6%, Dec - Jan 4.33%, Mar - Apr 4.33%, May - Jun 3.33%) but in geographic distribution (Jiangsu 6%, Anhui 5%, Zhejiang 2.5%). Regardless of isolation from different areas,swine and human genotype-IV HEV shared a high similarity. Phylogenetically, there were 80% - 100% and 96% - 100% identities within swine genotype-lV HEV at the nucleotide and amino acid levels respectively. Between swine HEV and human HEV, there were also similarities of 76% -99% and 95% - 100%. It was noted that some human and swine isolates were clustered with bootstrap values of > 90%.</p><p><b>CONCLUSION</b>Genotype-IV HEV is widely prevalent in swine herds in Eastern China and original common ancestor of evolution and transmission was implied. The sustaining prevalence within swine herds should have a probable influence on the epidemic situation of hepatitis E in human beings.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Filogenia
/
Estaciones del Año
/
Porcinos
/
Enfermedades de los Porcinos
/
Virología
/
Homología de Secuencia de Ácido Nucleico
/
China
/
Epidemiología
/
Prevalencia
/
Clasificación
Tipo de estudio:
Estudio de prevalencia
Límite:
Animales
/
Humanos
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
Chinese Journal of Preventive Medicine
Año:
2009
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS