Effects of tumor necrosis factor-alpha on the growth of rat osteoblasts / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of tumor necrosis factor alpha (TNF-alpha) on the growth of rat osteoblasts. To find out the mechanisms that TNF-alpha regulates the growth of osteoblasts.</p><p><b>METHODS</b>To assay osteoblasts proliferation by MTT. To assay alkaline phosphatase (ALP) activity of osteoblasts by PP-nitrophenyl phosphate (PNPP).</p><p><b>RESULTS</b>The osteblasts proliferation and the ALP activity decreased in treatment groups, and the significantly lower levels were observed in above 50 ng/mL groups (P<0.05).</p><p><b>CONCLUSION</b>TNF-alpha restrained osteoblasts proliferation and differentiation, and the effects were more significant in above 50 ng/mL groups.</p>