Comparison between MLO-Y4 osteocyte and osteoblast to support osteoclast formation in vitro / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To compare between MLO-Y4 osteocyte and osteoblast to support osteoclast formation in co-culture system.</p><p><b>METHODS</b>MLO-Y4 cells and murine osteoblast cells were co-cultured with bone marrow cells with or without vitamin D₃ presence.Bone marrow cells were as control group. Tartrat resistant acid phosphatase (TRAP)+ giant cells with three or more nuclei were counted and compared under a microscope at day 9.</p><p><b>RESULTS</b>In the absence of vitamin D₃, (1963.3 ± 93.1)/plate osteoclasts were observed when MLO-Y4 cells co-cultured with bone marrow cells in 24-well plate.While only (12.7 ± 5.5)/plate osteoclasts were found in the osteoblast group, and (6.0 ± 1.0)/plate in control group. The statistical difference occurs for any two groups (P < 0.05). Vitamin D₃ could significantly increase osteoclast formation in the three groups.</p><p><b>CONCLUSIONS</b>Osteocytes could induce osteoclastogenesis without the presence of vitamin D₃ and vitamin D₃ could enhance the induction effects of MLO-Y4 and osteoblast cells.</p>