Zinc finger protein-activating transcription factor up-regulates vascular endothelial growth factor-a expression in vitro / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 171-175, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-243243
ABSTRACT
<p><b>OBJECTIVE</b>To construct the zinc finger protein-activating transcription factor (ZFP-ATF) plasmid and evaluate its efficacy in inducing vascular endothelial growth factor (VEGF) expression in EY.HY926 endothelial cells.</p><p><b>METHODS</b>Firstly, we constructed the ZFP-ATF plasmid, then testified the quantity of VEGF protein in EY.HY926 endothelial cells after transfected with ZFP-ATP plasmid by Western blot, finally, we used the RT-PCR to testify whether the ZFP-ATF can stimulate expression of VEGF splice variants.</p><p><b>RESULTS</b>The ZFP-ATF DNA sequences were located the multiclone sites of PVAX1 vector between the site of BamH1and Xhol.Western blot result showed VEGF expression in EY.HY926 endothelial cells transfected with ZFP-ATF plasmid was significantly higher than that in cells transfected with VEGF165 (19.95±3.95 vs.12.15±1.55 μg÷μL, P<0.01).RT-PCR result showed VEGF-A mRNA expression level induced by ZFP-ATF was high than that induced by VEGF165.</p><p><b>CONCLUSION</b>ZFP-ATF can up-regulate the VEGF-A expression in comparison with VEGF165, which might have beneficial effects in angiogenesis process.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Plásmidos
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Regulación hacia Arriba
/
Células Cultivadas
/
Dedos de Zinc
/
Secuencia de Aminoácidos
/
Neovascularización Fisiológica
/
Células Endoteliales
Límite:
Humanos
Idioma:
Inglés
Revista:
Chinese Medical Sciences Journal
Año:
2012
Tipo del documento:
Artículo
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