Detection of deletional alpha-thalassemia from free fetal DNA in maternal plasma / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 679-682, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-243287
ABSTRACT
This study was aimed to develop a simple, rapid, accurate and wound-free single-tube multiplex polymerase chain reaction (PCR) assay, which can be used for molecular screening and prenatal diagnosis of 3 types of the commonest deletional alpha-thalassemias in Chinese population. The deletional alpha-thalassemias free fetal DNA of 50 anemic pregnant plasma were detected by means of single-tube multiplex PCR with 4 groups of primers designed by using the gap-PCR protocol. The results showed that 5 cases were found to be alpha-thalassemias in free fetal DNA of 50 anaemia pregnant plasma, out of them 3 cases were the Southeast Asia type of deletional alpha-thalassemias, 2 cases were alpha-(3.7) type of deletional alpha-thalassemias (rightward deletion). It is concluded that the single-tube multiplex PCR assay presented in this study is a simple and convenient, rapid and accurate method for detecting 3 common deletional alpha-thalassemias, and use of this method has an important significance for the deletional alpha-thalassemias to ensure the population health quality and reduce the social burden.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Diagnóstico Prenatal
/
ADN
/
Reacción en Cadena de la Polimerasa
/
Eliminación de Secuencia
/
Talasemia alfa
/
Cartilla de ADN
/
Diagnóstico
/
Feto
/
Genética
/
Métodos
Tipo de estudio:
Estudio diagnóstico
/
Guía de Práctica Clínica
Límite:
Femenino
/
Humanos
/
Embarazo
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2010
Tipo del documento:
Artículo
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