Preparation and antioxidant activity detection of collagen peptide from Cirrhinus molitorella skin / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1727-1734, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-243685
ABSTRACT
In order to prepare antioxidant peptide through hydrolyzing low-value protein resources with bacterial extracellular proteases and to discover novel proteases, crude extracellular protease from Pseudoalteromonas sp. SHK1-2 was obtained through fermentation which was used to hydrolyze collagen extracted from Cirrhinus molitorella skin. Small peptide fraction was isolated from hydrolysate by ultrafiltration and Sephadex LH-20 size exclusion chromatography and showed 1, 1-diphenyl-2-picrylhydrazyl radical scavenging activity (35.6%±7%), oxygen radical absorbance capacity and inhibition of DNA oxidation damage. The molecule weight was 776.2 Da, and amino acid sequence was Thr-Ala-Gly-His-Pro- Gly-Thr-His through liquid chromatography mass spectrum. Our findings suggest that peptide obtained from low-value protein of fish waste by hydrolysis with bacterial protease has antioxidant activity.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oxidación-Reducción
/
Péptido Hidrolasas
/
Péptidos
/
Piel
/
Cyprinidae
/
Química
/
Cromatografía en Gel
/
Colágeno
/
Secuencia de Aminoácidos
/
Dextranos
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2016
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS