High-dose heat shock protein gp96 immunization prevents type 1 diabetes via inducing regulatory T cells / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1685-1693, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-243689
ABSTRACT
Type 1 diabetes (T1D), the most prevalent human autoimmune disease, occurs in genetically susceptible individuals. Regulatory T cells (Tregs) are defective in T1D setting. Therefore, efforts to repair or restore Tregs in T1D may prevent or reverse this autoimmune disease. Here, we studied the potential role of rgp96 in preventing T1D, using non-obese diabetic (NOD) mice as an animal model. High-dose rgp96 immunization elicited efficient protection of mice against T1D, as evidenced by stable blood glucose, decreased disease incidence. Significantly increased CD4⁺ CD25⁺ Foxp3⁺ Tregs were observed in immunized mice. In vitro co-culture experiments demonstrated that rgp96 stimulation enhanced Treg proliferation and suppressive function by up-regulation of Foxp3 and IL-10. Our work shows that activation of Tregs by high-dose rgp96 immunization protects against T1D via inducing regulatory T cells and provides preventive and therapeutic potential for the development of an rgp96-based vaccine against T1D.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Terapéutica
/
Regulación hacia Arriba
/
Vacunación
/
Ratones Endogámicos NOD
/
Interleucina-10
/
Linfocitos T Reguladores
/
Técnicas de Cocultivo
/
Diabetes Mellitus Tipo 1
/
Alergia e Inmunología
/
Factores de Transcripción Forkhead
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2016
Tipo del documento:
Artículo
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