AcSDKP attenuates PDGF-induced cardiac fibroblasts proliferation and collagen expression: role of extracellular regulated protein kinase 1/2 pathway / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 444-448, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-243760
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of AcSDKP on platelet-derived growth factor (PDGF)-induced rat cardiac fibroblasts proliferation and collagen expression and explore the role of extracellular regulated protein kinase 1/2 (ERK1/2) pathway on this process.</p><p><b>METHODS</b>Metabolic activity of fibroblasts was determined by CCK-8. Cell cycle was detected by flow cytometry. Expressions of type I and type III collagen were measured by immunocytochemistry and Western blot. Expressions of phospho-ERK1/2 and ERK1/2 were detected by Western blot.</p><p><b>RESULT</b>10(-9) mol/L AcSDKP could significantly inhibit PDGF-induced cardiac fibroblasts proliferation, collagen expression and expressions of phospho-ERK1/2, while the protein levels of ERK1/2 were not significantly affected by AcSDKP.</p><p><b>CONCLUSION</b>AcSDKP could inhibit PDGF-induced cardiac fibroblasts proliferation and collagen expression through activation of phosphor-ERK1/2 pathway.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oligopéptidos
/
Fosforilación
/
Factor de Crecimiento Derivado de Plaquetas
/
Transducción de Señal
/
Células Cultivadas
/
Colágeno
/
Ratas Wistar
/
Proteína Quinasa 1 Activada por Mitógenos
/
Biología Celular
/
Miocitos Cardíacos
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Cardiology
Año:
2008
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS