Detection of SEN virus in sera of patients with chronic hepatitis B and general population in 5 cities of China / 中华流行病学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the prevalence of SEN virus (SENV) infection in CHB patients in five cities of China.</p><p><b>METHODS</b>A nest-polymerase chain reaction (nPCR) was used for detection of SENV-D and SENV-H in sera of 595 CHB patients from 5 cities of China and 96 normal individuals from Beijing. A total of 7 SENV strains were analyzed by direct sequencing.</p><p><b>RESULTS</b>The prevalence rates of SENV in CHB patients and normal individuals were 61.3% and 62.5%, respectively (chi(2) = 0.047, P = 0.829). The prevalence rates of CHB patients between 5 cities were different. Nucleotide sequence analysis showed that the homology between 4 SENV-D strains was 91% - 98% and 95% - 98% between 3 SENV-H strains isolated from 5 cities in China.</p><p><b>CONCLUSION</b>SENV-D/H were prevalent in CHB patients of China and their prevalence rates were similar to that in normal individuals.</p>