The three-dimensional culture of adult mesenchymal stem cells for intervertebral disc tissue engineering / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1300-1305, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-244639
ABSTRACT
Intervertebral disc (IVD) degeneration is one of the major causes of low back pain. As current clinical treatments are aimed at restoring biomechanical function and providing symptomatic relief, the methods focused on biological repair have aroused interest and several tissue engineering approaches using different cell types have been proposed. Owing to the unsuitable nature of degenerate cells for tissue engineering, attention has been given to the use of mesenchymal stem cells (MSCs). In this connection, we have made a study on the characteristics of MSCs derived from adult bone marrow and on the feasibility of constructing IVD tissue-engineering cell under a Three-Dimensional Pellet Culture System. The human bone marrow MSCs were isolated and purified with density gradient solution and attachment-independent culture system. MSCs isolated using this method are a homogeneous population as indicated by morphology and other criteria. They have the capacity for self-renewal and proliferation, and the multilineage potential to differentiate.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Terapéutica
/
Células de la Médula Ósea
/
Células Cultivadas
/
Técnicas de Cultivo de Célula
/
Condrogénesis
/
Biología Celular
/
Ingeniería de Tejidos
/
Degeneración del Disco Intervertebral
/
Células Madre Mesenquimatosas
Límite:
Adolescente
/
Adulto
/
Humanos
Idioma:
Chino
Revista:
Journal of Biomedical Engineering
Año:
2009
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS