Your browser doesn't support javascript.
loading
Expression of 11β-hydroxysteroid dehydrogenase type 2 in lymphoblastic cells and its relationship with glucocorticoid sensitivity / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 109-113, 2011.
Artículo en Chino | WPRIM | ID: wpr-244974
ABSTRACT
This study was aimed to explore the expression of 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) in 3 different lymphoblastic cell lines with relation to their glucocorticoid (GC) sensitivity. The 11β-HSD2 expressions in acute lymphoblastic leukemia Jurkat cells, lymphoma Daudi and Raji cells, and peripheral blood T cells of a healthy volunteer were analyzed by real time PCR and Western blot. Glucocorticoid (GC)-induced apoptosis in 3 different cell lines was detected by flow cytometry. Cell growth in Jurkat cells treated with cortisol was analyzed by trypan blue dye exclusion. Flow cytometry was performed to observe GC-induced apoptosis in Jurkat cells treated by combination of GC with 11β-HSD2 inhibition 18β-glycyrrhetinic acid (18β-GA). The results demonstrated that 11β-HSD2 highly expressed in Jurkat cells, but not in Daudi, Raji cells and normal blood T cells. Compared to Daudi and Raji cells, Jurkat cells were more resistant to GC-induced apoptosis. Furthermore, the inhibition of 11β-HSD2 by 18β-GA resulted in increased cellular sensitivity to GC as shown by elevated induction of apoptosis. it is concluded that 11β-HSD2 is at least partly responsible for GC resistance in Jurkat cells. 11β-HSD2 may be a potential target for reduction of GC-resistance in therapeutic applications.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Linfocitos / Células Jurkat / Línea Celular Tumoral / 11-beta-Hidroxiesteroide Deshidrogenasa de Tipo 2 / Glucocorticoides / Ácido Glicirretínico / Metabolismo Tipo de estudio: Estudio diagnóstico Límite: Humanos Idioma: Chino Revista: Journal of Experimental Hematology Año: 2011 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Linfocitos / Células Jurkat / Línea Celular Tumoral / 11-beta-Hidroxiesteroide Deshidrogenasa de Tipo 2 / Glucocorticoides / Ácido Glicirretínico / Metabolismo Tipo de estudio: Estudio diagnóstico Límite: Humanos Idioma: Chino Revista: Journal of Experimental Hematology Año: 2011 Tipo del documento: Artículo