Metabonomic study on the anti-liver injury effect of Si-Ni-San on rats by using UPLC-MS/MS / 药学学报
Acta Pharmaceutica Sinica
;
(12): 368-373, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-245075
ABSTRACT
A UPLC-MS/MS method based on metabonomic skills was developed to study the serum metabolic changes of rats after acute liver injury induced by CCl4 and to evaluate the action mechanism of Si-Ni-San. The integrated data were exported for principal components analysis (PCA) by using SIMCA-P software, in order to find the potential biomarkers. It showed that clear separation of healthy control group, model group, silymarin group, Si-Ni-San group was achieved by using the PCA method. Nine significantly changed metabolites were identified as potential biomarkers of acute liver injury. Compared with the health control group, the model group rats showed higher levels of phenylalanine, tryptophan and GCDCA together with lower levels of LPC 16 0, LPC 18 0, LPC 18 1, LPC 16 1, LPC 20 4 and LPC 22 6. These changes of serum metabolites suggested that the disorders of amino acid metabolism, lipid metabolism, bile acid biosynthesis and anti-oxidative damage were related to acute liver injury induced by CCl4. Si-Ni-San might have the anti-liver injury effect on all these four metabolic pathways.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Fenilalanina
/
Plantas Medicinales
/
Triptófano
/
Sangre
/
Medicamentos Herbarios Chinos
/
Intoxicación por Tetracloruro de Carbono
/
Lisofosfatidilcolinas
/
Distribución Aleatoria
/
Química
Límite:
Animales
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2014
Tipo del documento:
Artículo
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