Relationship between proteoglycans and proliferation of human salivary adencid cystic carcinoma / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 20-25, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-245252
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of down-regulated proteoglycans on the proliferation of human salivary adenoid cystic carcinoma (SACC).</p><p><b>METHODS</b>The short hairpin RNA (shRNA) plasmid silencing human xylosyltransferase-I (XT-I) gene was constructed and named shRNA-WJ3. Adenoid cystic carcinoma cells with high metastatic tendency (ACC-M) were transfected by shRNA-WJ3. The plasmid shRNA-HK not targeting any human gene was transfected into ACC-M cells used as negative control. After 48 h of transfection, the positive cells were screened by G418 to isolate the stable transfected cells. Real-time PCR and Western blotting were used to test the gene silence, and the proteoglycans contents of the cells were detected. The stable cell line silenced XT-I was named ACC-M-WJ3. MTT assay was performed to detect the cell proliferation. The cell cycle was analyzed by flow cytometry.</p><p><b>RESULTS</b>ShRNA-WJ3 showed powerful RNA interference and gene silence of XT-I. The inhibition rate was 83.70% of mRNA expression and 79.60% of protein expression respectively. The content of proteoglycans in ACC-M-WJ3 was down-regulated by 49.71%-54.59%. The results of MTT assay showed that the cell growth was inhibited significantly. S phrase decreased and G₁-G₀ phrase increased in group ACC-M-WJ3 compared with that of group ACC-M-HK (P < 0.05).</p><p><b>CONCLUSIONS</b>The down-regulated proteoglycans could inhibit the proliferation of human ACC-M cells.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Pentosiltransferasa
/
Proteoglicanos
/
ARN Mensajero
/
Neoplasias de las Glándulas Salivales
/
Transfección
/
Regulación hacia Abajo
/
Ciclo Celular
/
Carcinoma Adenoide Quístico
/
Silenciador del Gen
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Stomatology
Año:
2010
Tipo del documento:
Artículo
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