Dystrophin detection by immunofluorescent technique for diagnosing muscular dystrophy / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 239-242, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-245324
ABSTRACT
<p><b>OBJECTIVE</b>To establish a specific technique for diagnosing and classifying Duchenne muscular dystrophy (DMD), Becker muscular dystrophy (BMD), facioscapulohumeral muscular dystrophy (FSHD) and neurologic dystrophy.</p><p><b>METHODS</b>Forty-seven cases were detected by immunofluorescence technique for analyzing dystrophin located in skeletal muscle cell membrane with the use of mouse monoclonal antibodies, goat and rabbit polyclonal antibodies.</p><p><b>RESULTS</b>The normal individuals showed ringed positive staining stripe around muscle fibers. Negative result of staining was seen in 16 DMD patients. Eleven BMD patients had discontinuous or a patchy positive staining pattern, and all of 10 FSHD and 10 neurological amyotrophic patients showed positive dystrophin staining.</p><p><b>CONCLUSION</b>Detecting dystrophin in the skeletal muscle cell membrane of muscular patients is an efficient technique for diagnosing and classifying various types of muscular dystrophy.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Química
/
Distrofina
/
Técnica del Anticuerpo Fluorescente
/
Músculo Esquelético
/
Distrofia Muscular de Duchenne
/
Distrofia Muscular Facioescapulohumeral
/
Diagnóstico
/
Diagnóstico Diferencial
/
Metabolismo
Tipo de estudio:
Estudio diagnóstico
Límite:
Adolescente
/
Adulto
/
Niño
/
Child, preschool
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Medical Genetics
Año:
2002
Tipo del documento:
Artículo
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