Construction and identification of small interfering RNA expression plasmid targeting Sox9 and the function to cell growth and apoptosis of human chondrosarcoma cells HTB94 / 中华外科杂志
Chinese Journal of Surgery
;
(12): 772-775, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-245531
ABSTRACT
<p><b>OBJECTIVES</b>To construct small interfering (siRNA) Sox9 expression plasmid and transfer it into human chondrosarcoma cells HTB-94, and to check the mRNA and protein expression of Sox9 and cell growth and apoptosis of HTB-94 human chondrosarcoma cells.</p><p><b>METHODS</b>siRNA(Sox9) expression plasmid was designed and synthesized. And it was transferred into HTB-94 human chondrosarcoma cells. Then the expression of the mRNA and protein of Sox9, cell growth and apoptosis in transferred HTB-94 human chondrosarcoma cells were checked.</p><p><b>RESULTS</b>The recombinant plasmid was confirmed by enzyme digestion analysis and DNA sequencing. The expression of the mRNA and protein expression of Sox9 in transferred HTB-94 were significantly reduced. The cell growth of HTB-94 was inhibited, and the apoptosis of HTB-94 was remarkably increased.</p><p><b>CONCLUSION</b>siRNA (Sox9) expression plasmid could be transferred into HTB-94 human chondrosarcoma cells. And it can reduce the mRNA and protein expression of the HTB-94, inhibit the cell growth and cause the apoptosis of the tumor cells.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Plásmidos
/
ARN Mensajero
/
Células Tumorales Cultivadas
/
Transfección
/
Condrosarcoma
/
Apoptosis
/
ARN Interferente Pequeño
/
Proliferación Celular
/
Factor de Transcripción SOX9
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Surgery
Año:
2008
Tipo del documento:
Artículo
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