Inhibition of HCV replication by HCV specific U1 small nuclear RNA chimeric ribozyme in vivo / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 11-14, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-245765
ABSTRACT
<p><b>OBJECTIVE</b>To determine the advantage of U1 small nuclear RNA as a ribozyme vector (U1-Rz) to inhibit HCV replication in vivo.</p><p><b>METHODS</b>The 3rd stem-loop was substituted by HCV core specific ribozyme to construct an U1-Rz eucaryotic expression plasmid. Then it was co-transfected with pCMV/T7-NCRC Delta-luc into Huh7 cell line mediated by lipofectin. The cell lysis supernatant was subjected to Western blot and lumenometer to determine the luciferase levels.</p><p><b>RESULTS</b>A U1 snRNA chimeric ribozyme was constructed successfully. Both Rz and U1-Rz inhibited luciferase expression in Huh7 by 48.64% and 87.46%, respectively.</p><p><b>CONCLUSION</b>Rz has more efficacy in cells when using U1 snRNA delivery system. U1 can be an efficient vector for HCV specific ribozyme.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Replicación Viral
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Terapia Genética
/
ARN Nuclear Pequeño
/
ARN Catalítico
/
Hepacivirus
/
Vectores Genéticos
/
Genética
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Hepatology
Año:
2006
Tipo del documento:
Artículo
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