The direct antiviral effect of interferon-gamma on hepatitis C virus replicon / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 7-10, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-245766
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of interferon-gamma and interferon-gamma combined with interferon-alpha on HCV RNA replication and the possible mediators of interferon-gamma anti-HCV in vitro.</p><p><b>METHODS</b>An HCV replicon cell culture system was established and the cells were treated with interferon-gamma or interferon-gamma combined with interferon-alpha. HCV RNA levels in the cells were evaluated by semi-quantitative RT-PCR and real-time PCR, and the levels of NS5A protein were examined by Western blot.</p><p><b>RESULTS</b>Interferon-gamma could inhibit HCV RNA replication and NS5A protein expression effectively; The anti-HCV effects of interferon-gamma were both in time-dependent and does-dependent manners; Pretreating the cells with interferon-gamma could significantly enhance the antiviral effects of interferon-alpha; The expressions of IRF-1, 2'5'-OAS1(p46), 2'5'-OAS2(p69), ISGF3gamma and STAT1 were significantly increased after interferon-gamma treatment.</p><p><b>CONCLUSION</b>Interferon-gamma has a direct inhibitory effect on HCV replicon RNA replication and NS5A expression, and both are in a dose and time dependent manner; Interferon-gamma has a synergistic anti-HCV effect with interferon-alpha. IRF-1, 2'5'-OAS1(p46), 2'5'-OAS2(p69) and ISGF3gamma may mediate the anti-HCV effects of interferon-gamma.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Antivirales
/
Patología
/
Farmacología
/
Replicón
/
Replicación Viral
/
ARN Viral
/
Células Tumorales Cultivadas
/
Interferón gamma
/
Interferón-alfa
/
Hepacivirus
Límite:
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Hepatology
Año:
2006
Tipo del documento:
Artículo
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