Study on separation and purification of total alkaloids and mesaconitine with X - 5 macroporous resin / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 396-400, 2007.
Article
en Zh
| WPRIM
| ID: wpr-245968
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study systematically the factors which affect separation and purification of the total alkaloids and mesaconitine with X -5 macroporous resin.</p><p><b>METHOD</b>With the content of the total alkaloids and mesaconitine as parameters, the optimum condition of absorption and elution were studied in the process of the purification with X -5 macroporous resin.</p><p><b>RESULT</b>The X - 5 macroporous resin yielded the best separating efficiency when the concentration of the extracted solution was 1 g raw material per 1 mL, pH 12.0, the absorptive time of 6 hour and the volume of 95% ethanol (7BV pH 8) as the eluant; X -5 macroporous resins was used five times in a reproducible way. The rate of extraction and content of the total alkaloid were 80% and 30% respectively after purification with X - 5 macroprous resin.</p><p><b>CONCLUSION</b>The method can increase the purity of mesaconitine and total alkaloids.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Plantas Medicinales
/
Resinas Sintéticas
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Aconitina
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Química
/
Raíces de Plantas
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Aconitum
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Etanol
/
Alcaloides
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Concentración de Iones de Hidrógeno
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2007
Tipo del documento:
Article