Effects of methyl jasmonat on accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy root / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 300-302, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-245989
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of methyl jasmonate (MJ) on the accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy roots.</p><p><b>METHOD</b>After 18 day's suspension culture of S. miltiorrhiza hairy roots induced by Agrobacterium rhizogenes ATCC15834, the chemical elicitor--methyl jasmonat was added into 6-7V suspension cultures and at the same time, tanshinones contents (including cryptotanshinone and tanshinone II(A)) on the day 2, 6 and 9, after dealing with MJ, was quantified by HPLC.</p><p><b>RESULT</b>After dealing with MJ on the day 2, 6 and 9, the concentration of cryptotanshinone reached to 0.039, 0.204, 0.571 mg x g(-1) respectively,and tanshinone II(A) reached 0.251, 0.601 and 1.563 mg x g(-1) respectively. After 9 day's treatment by MJ, the maximum increase of cryptotanshinone and tanshinone II(A) were 23.8 fold and 6.2 fold higher than that of the control respectively.</p><p><b>CONCLUSION</b>MJ could stimulate the accumulation of tanshinones in S. miltiorrhiza and have released them into the culture medium.</p>
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WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Fenantrenos
/
Reguladores del Crecimiento de las Plantas
/
Plantas Medicinales
/
Raíces de Plantas
/
Ciclopentanos
/
Salvia miltiorrhiza
/
Abietanos
/
Técnicas de Cultivo
/
Oxilipinas
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2007
Tipo del documento:
Artículo
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