Determination of bufothionine in skin of Bufo bufo gargarizans and Huachansu injection / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 224-226, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-246004
ABSTRACT
<p><b>OBJECTIVE</b>To establish a HPLC method for bufothionine in the skin of Bufo bufo gargarizans and Huachansu injection.</p><p><b>METHOD</b>The samples were separated using a Lichrosob C18 column with CH3CN-H2O (1090) as mobile phase. Flow rate was at 1.0 mL x min (-1) and the detection wavelength was at 225 nm.</p><p><b>RESULT</b>The calibration curve of bufothionine was linear over the range of 0.0772-0.4632 microg and the average recovery was 99. 2%. The contents of bufothionine were fluctuated from 36.4-641.8 microg x g(-1) in the skin of Bufo bufo gargarizans and 22.47-33.16 microg x mL(-1) in Huachansu injection, respectively.</p><p><b>CONCLUSION</b>The contents of bufothionine were greatly different between cultured and wild species. The method was suitable for the quality control of the skin of Bufo bufo gargarizans and its preparation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fenotiazinas
/
Control de Calidad
/
Piel
/
Bufo bufo
/
Materia Medica
/
Química
/
China
/
Reproducibilidad de los Resultados
/
Cromatografía Líquida de Alta Presión
/
Inyecciones
Límite:
Animales
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2007
Tipo del documento:
Artículo
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