Research on Sailong boneextracts on proliferation and apoptosis of osteoblast cells / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1991-1994, 2006.
Article
en Zh
| WPRIM
| ID: wpr-246033
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the metabolic regulation and apoptosis of Sailong bone extracts on rat osteoblast cells in vitro.</p><p><b>METHOD</b>Sailong bone fat-soluble extract, Sailong bone ethanol extract and Sailong bone aqueous extract were extracted with super critical fluid extraction (SCFE) , and Sailong bone boiling water component was extracted with distilled water directly. MTT assay was applied to determine the proliferation of the cell promoted by four Sailong bone extracts and PAS assay for the aqueous proportion of the cell at different doses.</p><p><b>RESULT</b>Sailong bone fat-soluble and aqueous extract (each 10 mg x mL (-1)) could significantly improve the proliferation of rat osteoblast cells ROS 17/2. 8 (P < 0. 01). Compared with the blank, the proportion of xub-G, of the different extracts from Sailong bone is reduce evidently. The result have shown the extracts from Sailong bone could reduce the rate of aqueous of cell and could suspend the aqueous.</p><p><b>CONCLUSION</b>Sailong bone can promoting the proliferation, degrading the rate of the apoptosis and delay the development of osteoblast to be the substitute of the bone of tiger as a Chinese materia medica.</p>
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Índice:
WPRIM
Asunto principal:
Osteoblastos
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Farmacología
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Roedores
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Factores de Tiempo
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Huesos
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Materia Medica
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Células Cultivadas
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Química
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Apoptosis
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Biología Celular
Límite:
Animals
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2006
Tipo del documento:
Article