Study on extraction process of polysaccharide from Sargassum fusiforme by enzymatic treatment / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the optimal extraction process of polysaccharides from S. fusiforme by enzymatic treatment.</p><p><b>METHOD</b>The optimum extraction conditions were obtained by the experiment with the orthogonal design. The content of polysaccharides of S. fusiforme was determined by spectraphotometry.</p><p><b>RESULT</b>The amount of enzyme and temperature significantly affected total polysaccharides of S. fusiforme.</p><p><b>CONCLUSION</b>The optimum extraction conditions include the addition of 1. 2 x 10 (4) U x 100 g(-1) enzyme into water at pH 4. 5, and the subsequent treatment for 10 min while the temperature is maintained at 45 degrees C.</p>