Dynamic expression of TGF-beta1/Smad protein in CCl4-induced liver fibrosis and its significance in rats / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 334-337, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-246247
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the dynamic expression of TGF-beta1/Smad protein in rats with liver fibrosis induced by carbon tetrachloride (CCl4), to study mechanism of TGF-beta1/Smad signaling and the relationship between its transduction and liver fibrosis.</p><p><b>METHODS</b>Fifty healthy male SD rats were randomly divided into two groups normal control group and model group. Experimental liver fibrosis was induced by subcutaneous injection of CCl4. After six weeks and nine weeks, histopathological changes and degrees of fibrosis were observed by optical microscopy. Meanwhile, the expression of TGF-beta1, TP3R-I, Smad2/3 and Smad7 proteins was detected by immunohistochemistry.</p><p><b>RESULTS</b>(1) Pathological observation of hepatic specimens hepatic tissue of model group rat had inflammation and fibrosis in different degrees. By comparing with the degrees of inflammation and fibrosis model groups were more severe than normal control group (P < 0.05). (2) Changes of protein levels about TGF-beta1/Smad the expressions of TGF-beta1, TbetaR-I, Smad2/3 and Smad7 in rat hepatic tissue were detected with immunohistochemistry techniques. The expressions of the four items in model group were higher than those of normal control group (P < 0.01). In fibrosis model group, there exist considerable positive correlations among expressions of TGF-beta1, TbetaR-I, Smad2/3, Smad7 and degrees of fibrosis in livers (P < 0.05 or 0.01).</p><p><b>CONCLUSION</b>There is close relation between the level of TGF-beta1, TbetaR-I, Smad2/3, Smad7 and the different liver fibrosis grades due to CCl4. The up regulation of TGF-beta1, TbetaR- I, Smad2/3 and Smad7 in liver tissue is involved in the progression of hepatic fibrosis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Tetracloruro de Carbono
/
Distribución Aleatoria
/
Regulación hacia Arriba
/
Ratas Sprague-Dawley
/
Modelos Animales de Enfermedad
/
Proteína smad7
/
Proteína Smad2
/
Proteína smad3
/
Factor de Crecimiento Transformador beta1
/
Genética
Tipo de estudio:
Ensayo Clínico Controlado
/
Estudio pronóstico
Límite:
Animales
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2011
Tipo del documento:
Artículo
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