Expression of corticotropin-releasing hormone receptor type-1 in intrahepatic cholestatic placental tissue / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 381-384, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-246677
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression level of corticotropin-releasing hormone receptor type-1 (CRHR-1) in intrahepatic cholestatic placental (ICP) tissue.</p><p><b>METHODS</b>Human placental samples were collected from 10 ICP patients and 10 healthy controls after parturition at 37-40 weeks of gestation. CRHR-1 protein and mRNA expression was assessed by western blotting and nested-real-time fluorescence quantitative PCR, respectively. Normally distributed data were summarized as mean +/- standard deviation, and intergroup comparisons were made by two-tailed Student's t-test. Non-normally distributed data were presented as median with interquartile range, and intergroup comparisons were made by Wilcoxon test. For all statistical analyses, a two-tailed P-value of less than 0.05 was considered statistically significant.</p><p><b>RESULTS</b>The CRHR-1 fluorescence intensity was lower in ICP tissues (1.55 +/- 0.28) than in placental tissues from healthy controls (1.60 +/- 0.37), but the difference did not reach statistical significance (t = 0.349, P = 0.732). The CRHR-1 mRNA content was slightly higher in the ICP tissues [0.139(0.268)] than in the placental tissues from healthy controls [0.031(0.245)], but the difference did not reach statistical significance (t = 1.504, P = 0.136).</p><p><b>CONCLUSION</b>CRHR-1 expression is decreased in ICP tissues, which may lead to a smaller volume of placental lobular villi vessels and restrict the CRH positive feedback loop, ultimately promoting acute hypoxic stress and possible harm to the fetus.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Placenta
/
Complicaciones del Embarazo
/
Estudios de Casos y Controles
/
Colestasis Intrahepática
/
Receptores de Hormona Liberadora de Corticotropina
/
Cirrosis Hepática Biliar
/
Metabolismo
Tipo de estudio:
Estudio observacional
Límite:
Adulto
/
Femenino
/
Humanos
/
Embarazo
Idioma:
Chino
Revista:
Chinese Journal of Hepatology
Año:
2013
Tipo del documento:
Artículo
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