B-1 Cells Differ from Conventional B (B-2) Cells: Difference in Proliferation
Immune Network
;
: 155-160, 2004.
Artículo
en Coreano
| WPRIM
| ID: wpr-24702
ABSTRACT
BACKGROUND:
B-1 cells differ from conventional B-2 cells both phenotypically and functionally. The aim of this study was to investigate the difference between peritoneal B-1 cells and splenic B-2 cells in proliferation.METHODS:
We obtained sorted B-1 cells from peritoneal fluid and B-2 cells from spleens of mice. During the culture of these cells, immunoglobulin secreted into the culture supernatants was evaluated by enzyme- linked immunosorbent assay. Entering of S phase in response to LPS-stimuli was measured by proliferative assay.RESULTS:
Spontaneous Immunoglobulin M production occurred in peritoneal B-1 cells but not in splenic B-2 cells. LPS stimulated peritoneal B-1 cells secreted IgM at day 1, but splenic B-2 cells at day 2. In thymidine incorporation, peritoneal B-1 cells entered actively S phase after 24hours LPS-stimulation but splenic B-2 cells entered actively S phase after 48 hours.CONCLUSION:
IgM secretion and S phase entering occurred early in peritoneal B-1 cells compared to splenic B-2 cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Bazo
/
Timidina
/
Líquido Ascítico
/
Inmunoglobulina M
/
Inmunoglobulinas
/
Fase S
Límite:
Animales
Idioma:
Coreano
Revista:
Immune Network
Año:
2004
Tipo del documento:
Artículo
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