The development of a fluorescence polarization immunoassay for aflatoxin detection / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 126-129, 2014.
Artículo
en Inglés
| WPRIM
| ID: wpr-247074
ABSTRACT
A fluorescence polarization immunoassay (FPIA) was developed for the analysis ofaflatoxins (AFs) using an anti-aflatoxin B1 (AFB1) monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed an IC50 value of 23.33 ng/mL with a limit of detection of 13.12 ng/mL for AFB1. The cross-reactivities of AFB1, AFB2, AFG1, AFG2, AFM1, and AFM2 with the antibody were 100%, 65.7%, 143%, 23.5%, 111.4%, and 2%, respectively. The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs, albeit not AFG2 and AFM2. The total time required for analyzing 96 samples in one microplate was less than 5 min. This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Inmunoensayo de Polarización Fluorescente
/
Aflatoxinas
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Biomedical and Environmental Sciences
Año:
2014
Tipo del documento:
Artículo
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