Rapid detection of chromosomes X and Y using dual-color primed in situ labeling technique / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop a rapid method for detection of chromosomes, and to present a feasible method for rapid detection of the interphase nuclei of uncultured amniocytes using modified dual-color primed in situ labeling (PRINS) technique.</p><p><b>METHODS</b>Chromosomes X and Y were detected and analyzed by the modified dual-color PRINS in 205 amniotic fluid samples.</p><p><b>RESULTS</b>The specific chromosomes were obtained on both metaphase and interphase nuclei. In more than 98% of the samples PRINS reactions with primers X and Y were successfully induced. One sample was properly identified and correctly scored as 47, XXY.</p><p><b>CONCLUSION</b>The results suggest that PRINS technique is rapid, simple and cost-effective. It is also sensitive and specific, and thus useful for rapid detection of chromosome abnormalities.</p>