Application of real-time fluorescence quantitative PCR accompanied with comparison of Delta CT for diagnosis of Down's syndrome from a single cell / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 200-202, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-247353
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the use of real-time fluorescence quantitative PCR (FQ-PCR) accompanied with comparison of Delta CT as a method for diagnosis of Down's syndrome from a single cell.</p><p><b>METHODS</b>Single lymphocyte was isolated from the peripheral or umbilical cord blood samples of 22 clinically diagnosed Down's syndrome patients or fetus and 40 normal controls by micromanipulation techniques. Primer extension preamplification (PEP) and real-time FQ-PCR were used to amplify the S100B and DCSR1 located on chromosome 21, and GAPDH located on chromosome 12. Two pairs of DeltaCT values were compared between the two groups. The ratios of S100B/GAPDH and DSCR1/GAPDH products were calculated in trisomy 21 group.</p><p><b>RESULTS</b>The DeltaCT values of Down's syndrome patients were significantly lower than those of normal controls. The difference between the two groups was statistically significant (P<0.01). The ratios of S100B/GAPDH and DSCR1/GAPDH products for trisomy 21 were 1.891(1.563-2.287) and 1.840 (1.562-2.168), respectively.</p><p><b>CONCLUSION</b>Real-time FQ-PCR is a reliabe method that may provide a new way for non-invasive prenatal diagnosis and preimplantation genetic diagnosis for Down's syndrome.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Diagnóstico Prenatal
/
Sangre
/
Linfocitos
/
Reacción en Cadena de la Polimerasa
/
Reproducibilidad de los Resultados
/
Sensibilidad y Especificidad
/
Síndrome de Down
/
Repeticiones de Microsatélite
/
Biología Celular
/
Diagnóstico
Tipo de estudio:
Estudio diagnóstico
Límite:
Child, preschool
/
Femenino
/
Humanos
/
Lactante
/
Masculino
/
Embarazo
Idioma:
Chino
Revista:
Chinese Journal of Medical Genetics
Año:
2007
Tipo del documento:
Artículo
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