Studies on anti-tumor metastatic constituents from Ardisia Crenata / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the anti-tumor metastatic constituents from Ardisia Crenata.</p><p><b>METHOD</b>Chemical constituents were isolated and purified by repeated column chromatography( silica gel, Toyopearl HW40C and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The anti-tumor metastasis assay was applied to evaluate the isolated compounds of their activities.</p><p><b>RESULT</b>Nine compounds(1-9) were isolated and their structures were identified by comparison of their spectral data with literature values as follows 5-hydroxymethyl-2-furalclehyde(1), ethyl-beta-D-fructopyranoside(2), syringic acid(3), n-butyl-beta-D-fructofuranoside(4), n-butyl-alpha-D-fructofuranoside(5), methyl-alpha-D-fructofuranoside(6), (+)-bergenin(7), ardisiacrispins B(8), asperuloside acid(9). The isolated compounds(1-9) showed positive anti-tumor metastatic activities, and compounds 1, 5, and 8 showed significant anti-tumor metastatic activities. At the concentration of 0.8 mg x L(-1), compound 5 revealed the value of metastatic inhibition ratio on MDA-MB-231 was 93.8%.</p><p><b>CONCLUSION</b>Compounds 2-6 and 9 were isolated from this plant for the first time. compounds 1, 5 and 8 showed significant anti-tumor metastatic activities.</p>