Characteristic chromatographic profile of Paeoniae Radix and its application in quality control of crude and processed drugs of different origins and processing methods / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a HPLC method of characteristic chromatographic profile for the quality control of Paeoniae Radix.</p><p><b>METHOD</b>The 67 batches of samples were analyzed on a Polaris C18-A column with a gradient elution of acetonitrile and phosphate solution (pH 3.0) at a flow rate of 1.0 mL x min(-1) and detected at 230 nm.</p><p><b>RESULT</b>Nine main marker peaks were identified and semi-quantificated. By the similarity evaluation software for chromatographic fingerprint of traditional Chinese medicine (Version 2004A) and hierarchical clustering analysis, 67 batches of Paeoniae Radix were classified.</p><p><b>CONCLUSION</b>The method can be applied for quality assessment of Paeoniae Radix.</p>