Simultaneous determination of five glycosides in Aidi injection by RP-HPLC-ELSD / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 706-708, 2011.
Article
en Zh
| WPRIM
| ID: wpr-247404
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a RP-HPLC-ELSD method for the simultaneous determination of five glycosides in Aidi injection.</p><p><b>METHOD</b>The separation was carried out at 35 degrees C on a Kromasil C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitrile and water as the mobile phases in gradient mode. The flow rate was 1.0 mL x min(-1). The SHIMADZU ELSD-LT II detector was used. The detector temperature was at 40 degrees C and the pressure of carrier gas N2 was 0.35 MPa.</p><p><b>RESULT</b>The linear response (the natural logarithm of peak areas with corresponding mass) ranges were 21.2-212 mg x L(-1) for syringin, 30.8-308 mg x L(-1) for ginsenoside Rg1, 24.8-248 mg x L(-1) for ginsenoside Re, 25.6-256 mg x L(-1) for ginsenoside Rb1, 31.2-312 mg x L(-1), for astragaloside IV, respectively. The average recoveries (n = 9) of five glycosides were greater than 97.6%, and RSD were less than 1.1%.</p><p><b>CONCLUSION</b>The results demonstrated that this method had adequate accuracy and selectivity to measure the concentrations of five glycosides in Aidi injection.</p>
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Asunto principal:
Fenilpropionatos
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Saponinas
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Triterpenos
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Medicamentos Herbarios Chinos
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Cromatografía Líquida de Alta Presión
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Ginsenósidos
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Glucósidos
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Glicósidos
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Inyecciones
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Métodos
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2011
Tipo del documento:
Article