Overexpression of adiponectin prevents hepatocyte steatosis / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 213-216, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-247555
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of adiponectin on hepatocyte steatosis.</p><p><b>METHODS</b>L02 cells were transfected with pEGFP-N1-AdipoQ, a plasmid encoding pEGFP-adiponectin fusion protein, or pEGFP-N1. Lipid droplets in the hepatocytes were observed by oil red staining at 72 h. The contents of TG, FFA and glycerol in hepatocytes were measured.</p><p><b>RESULTS</b>Compared to cells transfected with pEGFP-N1-AdipoQ plasmid, much more lipid droplets were observed in cells transfected with pEGFP-N1 plasmid. TG, FFA and glycerol contents in L02 cells and L02/pEGFP-N1 cells were significantly higher than those in L02/pEGFP-N1-AdipoQ cells.</p><p><b>CONCLUSIONS</b>Overexpression of adiponectin prevent hepatocyte steatosis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plásmidos
/
Triglicéridos
/
Proteínas Recombinantes de Fusión
/
Transfección
/
Línea Celular
/
Hepatocitos
/
Biología Celular
/
Adiponectina
/
Ácidos Grasos no Esterificados
/
Hígado Graso
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Hepatology
Año:
2010
Tipo del documento:
Artículo
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