Isolation and identification of dog periodontal ligament stem cells / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 79-83, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-248304
ABSTRACT
<p><b>OBJECTIVE</b>To isolate, culture and identify a dog periodontal ligament stem cells (PDLSC) line in vitro.</p><p><b>METHODS</b>The adult dog periodontal ligament cells were isolated by limited dilution of culture cell for single cell clone. Cells originated from one of these clones were assessed through colony-forming efficiency and immunocytochemistry assay and alkaline phosphatase stain was used to identify the source of adult dog periodontal stem cells, at the same time, PDLSC were induced with mineralizatin solution and was found to have long protrude like an osteoblast. Differentiation of PDLSC were assessed. Mineralized potential was studied by Von-Kossa staining.</p><p><b>RESULTS</b>The dog PDLSC expressed STRO-1, which was the marker of mesenchymal stem cells. Also Vimentin, osteoblast-like marker alkaline phosphatase and Collagen-I expressed weakly. Cells were clonegenic, highly proliferative cells and capable of differentiating into osteoblasts/cementoblasts.</p><p><b>CONCLUSION</b>The evidence suggests that the cultured cells were stem cells from adult dog periodontal ligament.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoblastos
/
Ligamento Periodontal
/
Células Madre
/
Diferenciación Celular
/
Células Cultivadas
/
Técnicas de Cultivo de Célula
/
Cemento Dental
/
Fosfatasa Alcalina
/
Células Madre Adultas
/
Células Madre Mesenquimatosas
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
West China Journal of Stomatology
Año:
2009
Tipo del documento:
Artículo
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