The surface antigen expression of periodontal ligament cells and dental pulp cells in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 20-23, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-248319
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of surface antigen of human periodontal ligament cells (PDLCs) and dental pulp cells (DPCs) and the impact of ex vivo expansion to the expression of surface antigen. To provide basis of proper surface antigen for further selection of homogenous stem cell subpopulation from PDLCs and DPCs.</p><p><b>METHODS</b>PDLCs and DPCs were isolated and cultured by collagenase type I and dispase. The expression of surface antigen was analyzed by immunohistochemistry and flow cytometry.</p><p><b>RESULTS</b>Positive expression of STRO-1 and CD146 were observed in PDLCs and DPCs by immunocytochemistry. Similar to DPCs, PDLCs expressed mesenchymal stem cell markers STRO-1, CD146, CD29, CD44 and CD106, and displayed negative expression for CD34 at passage 1 by flow cytometry. There were no significant difference of STRO-1, CD29 and CD44 expression level between PDLCs and DPCs (P > 0.05). PDLCs expressed significantly higher level of CD106 and significantly lower level of CD146 than DPCs (P < 0.001). The proportion of STRO-1 and CD146 positive cells decreased steadily with passages in PDLCs and DPCs.</p><p><b>CONCLUSION</b>PDLCs have some similar surface antigen as DPCs, and the stem cells properties of PDLCs and DPCs decreased steadily with passages.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ligamento Periodontal
/
Técnicas In Vitro
/
Células Cultivadas
/
Pulpa Dental
/
Células Epiteliales
/
Células Madre Mesenquimatosas
/
Citometría de Flujo
/
Antígenos de Superficie
Límite:
Humanos
Idioma:
Chino
Revista:
West China Journal of Stomatology
Año:
2009
Tipo del documento:
Artículo
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