A novel member of SH(2) signaling protein family: cloning and characterization of SH(2)A gene / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>Cloning and characterization of a novel gene by exon trapping and exon linking at chromosome 8p22.</p><p><b>METHODS</b>A novel gene was cloned using exon trapping and exon linking, and its expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot.</p><p><b>RESULTS</b>A sequence containing 3 exons was found. The sequence is homologous with the putative gene AK024799 which consists of 2880 bp cDNA with 1362 bp open reading frame and codes 454 amino acids with an SH(2) domain. The gene was named SH(2)A at chromosome 8p22. SH(2)A gene is ubiquitously expressed in various tissues with three transcripts. The aberrant expression of SH(2)A gene in some cancers was detected.</p><p><b>CONCLUSION</b>SH(2)A is a novel docking protein of SH(2) signaling protein family, which may play an important role in cellular signal transduction. It relates to the pathogenesis of tumor.</p>