Development and application of a two-step MacELISA for the early diagnosis of hemorrhagic fever with renal syndrome / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 6-8, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-248739
ABSTRACT
<p><b>OBJECTIVE</b>To develop and improve a MacELISA method for the early diagnosis of hemorrhagic fever with renal syndrome (HFRS) with simplified operation procedure.</p><p><b>METHODS</b>The nucleic proteins of hantavirus were labeled with horse raddish peroxidase (HRP) and used as detection antigens. A two-step MacELISA based HRP conjugated antigen was established and the detection sensitivity and specificity were compared with commonly used three-step MacELISA.</p><p><b>RESULTS</b>This method could be used to detect hantanvirus specific IgM with high sensitivity and specificity from human patient serum. There was not significant difference from commonly used three-step MacELISA and the sensitivity and specificity were 100%.</p><p><b>CONCLUSION</b>This method is simple, sensitive and rapid in operation, and therefore could be used for the early diagnosis of HFRS.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Factores de Tiempo
/
Sangre
/
Inmunoglobulina M
/
Ensayo de Inmunoadsorción Enzimática
/
Estudios de Casos y Controles
/
Reproducibilidad de los Resultados
/
Sensibilidad y Especificidad
/
Diagnóstico
/
Fiebre Hemorrágica con Síndrome Renal
/
Métodos
Tipo de estudio:
Estudio diagnóstico
/
Estudio observacional
/
Estudio de tamizaje
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2008
Tipo del documento:
Artículo
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