Preliminary analyses for influence of mutant PrPs with different number of octapeptide / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 208-210, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-248802
ABSTRACT
<p><b>OBJECTIVE</b>The present study was conducted to understand the effects of PrP in different octapeptide repeats on proliferation of HeLa cells.</p><p><b>METHODS AND RESULTS</b>Mutant PrPs with octapeptide repeat insertion were transiently expressed in HeLa cells and their results of MTT assay showed stronger cytotoxic effect on the proliferation of cells than wild-type PrP. Annexin V/PI assay also demonstrated that the expression of mutant PrPs was much easier to induce apoptosis than wild-type in HeLa cells. The percentage of both early and late stage apoptosis in mutant groups were significantly higher than that of wild type.</p><p><b>CONCLUSION</b>These data suggest that the expression of mutant PrPs associated with familial CJD is much easier to induce apoptosis in cultured cells than expression of wild type PrP.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oligopéptidos
/
Fisiología
/
Plásmidos
/
Priones
/
Células HeLa
/
Transfección
/
Supervivencia Celular
/
Western Blotting
/
Apoptosis
/
Colorimetría
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2007
Tipo del documento:
Artículo
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