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Starvation-induced autophagy in cultured non-small cell lung cancer cells / 南方医科大学学报
Journal of Southern Medical University ; (12): 627-630, 2014.
Artículo en Chino | WPRIM | ID: wpr-249393
ABSTRACT
<p><b>OBJECTIVE</b>To observe autophagy induced by starvation in non-small cell lung cancer A459 and 95D cells.</p><p><b>METHODS</b>A549 and 95D cells in logarithmic growth in 1640 medium were cultured in Earle's balanced salt solution (EBSS) for 0, 1, 2, 3, 4 or 5 h. Autophagosome formation in the cell culture was observed by MDC fluorescent staining, and the expression of microtubule-associated protein 1 light chain 3 (LC3) and Beclin1 in the cells were detected using Western blotting.</p><p><b>RESULTS</b>Compared with the control cells, the cells with prolonged starvation showed increased MDC-positive cells and autophagosome formation. The expression of Beclin-1 and the LC3-II/LC3-I ratio also increased as the starvation prolonged, reaching the peak levels at 3 h and 4 h, respectively.</p><p><b>CONCLUSION</b>Autophagy can be induced by starvation in A549 and 95D cells in correlation with the expression of autophagy-related proteins LC3 and Beclin-1. These cell models of nutritional deficiency-induced autophagy may allow for a better understanding of the role of autophagy in the development of non-small cell lung cancer.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Autofagia / Carcinoma de Pulmón de Células no Pequeñas / Línea Celular Tumoral / Proteínas Reguladoras de la Apoptosis / Beclina-1 / Proteínas de la Membrana / Metabolismo / Proteínas Asociadas a Microtúbulos Tipo de estudio: Estudio pronóstico Límite: Humanos Idioma: Chino Revista: Journal of Southern Medical University Año: 2014 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Autofagia / Carcinoma de Pulmón de Células no Pequeñas / Línea Celular Tumoral / Proteínas Reguladoras de la Apoptosis / Beclina-1 / Proteínas de la Membrana / Metabolismo / Proteínas Asociadas a Microtúbulos Tipo de estudio: Estudio pronóstico Límite: Humanos Idioma: Chino Revista: Journal of Southern Medical University Año: 2014 Tipo del documento: Artículo