An initial investigation on the in vitro culture system of primordial germ cells in golden hamsters / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 601-604, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-249547
ABSTRACT
To establish the in vitro culture system of primordial germ cells (PGCs) of golden hamsters, PGCs of hamster were isolated from genital ridge of embryos at 10. 5th dpc (day post coitum), obtained by enzyme-mechanical method, and cultured on feeder cells. Then the PGCs were identified by alkaline phosphatase (ALP) activity staining. In order to induce the PGCs to differentiate in vitro, we removed the differential inhibition factors in the conditioned medium and observed the formation of embryoids and differentiated cells from three layers. The result showed that the pluripotent primordial germ cells could be successfully obtained from the golden hamsters at 10. 5th dpc by the enzyme-mechanical method and that PGCs were identified by both their strong positive reaction in ALP activity staining test and their differentiation into three-layer derived cells in vitro. The result suggests that the establishment of in vitro PGCs culture system of golden hamsters will provide new cell source for biomedical engineering.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Diferenciación Celular
/
Células Cultivadas
/
Mesocricetus
/
Técnicas de Cultivo de Célula
/
Biología Celular
/
Embrión de Mamíferos
/
Células Germinativas
/
Métodos
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Biomedical Engineering
Año:
2006
Tipo del documento:
Artículo
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