Expression of the infectious bursal disease virus polyprotein in Vero cells using attenuated Salmonella typhimurium as transgenic carrier / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 437-440, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-249967
ABSTRACT
To examine if polyprotein gene (VP2/VP4/VP3) of Infectious Bursal Disease Virus (IBDV) could be delivered into mammalian cells and expressed using attenuated Salmonella typhimurium as vector. The IBDV polyprotein gene was amplified by RT-PCR and inserted in to pCI, an eukaryotic expression plasmid. The resulting recombinant pCI-VP2/VP4/VP3 was transformed by electroporation into attenuated Salmonella typhimurium strain ZJ111 (dam- and phoP-), which was then use to transfect the Vero cells. Gene specific RT-PCR revealed that VP2/VP4/VP3 was transcribed into mRNA in the Vero cells. Indirect immunofluorscence assay, SDS-PAGE and Western-blot analysis showed that VP2/VP4/VP3 was expressed and the product was immuno-reactive with anti-IBDV serum. This work provides essential precondition for developing a new oral DNA vaccine against IBDV.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Salmonella typhimurium
/
Proteínas Virales
/
Células Vero
/
Transfección
/
Chlorocebus aethiops
/
Electroporación
/
Virus de la Enfermedad Infecciosa de la Bolsa
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Poliproteínas
/
Vectores Genéticos
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2004
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS