Effect of beta-catenin on the activation of hepatic stellate cells induced by transforming growth factor-beta1 / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 188-192, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-250017
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of beta-catenin on the activation of hepatic fibrosis by transforming growth factor-beta 1 (TGFbeta1).</p><p><b>METHODS</b>The recombinant expression plasmids pcDNA3.1(+)-beta-catenin and pEGFP-N1 were cotransfected into cultured HSC-T6 cells. The expression of smad3, beta-catenin and alpha-SMA, beta-catenin protein in TGFbeta1 treated HSC-T6 cells were detected by RT-PCR and Western-blot.</p><p><b>RESULTS</b>The expression of smad3 and beta-catenin in the co-transfected cells was higher than that in the untransfected cells (smad3 mRNA were 0.642 +/- 0.011, 0.501 +/- 0.021, 0.511 +/- 0.019, 0.356 +/- 0.017, respectively, F = 135.304, P < than 0.05. beta-catenin mRNA were 0.783 +/- 0.021, 0.543 +/- 0.033, 0.538 +/- 0.024, 0.212 +/- 0.019, respectively, F = 267.340, P < than 0.05. smad3 protein were 0.892 +/- 0.012, 0.124 +/- 0.011, 0.130 +/- 0.021, 0.003 +/- 0.001, F = 2823.813, P < l than 0.05. beta-catenin protein were 0.921 +/- 0.020, 0.210 +/- 0.010, 0.208 +/- 0.008, 0.002 +/- 0.001, respectively, F = 3440.982, P < than 0.05). The expression of beta-catenin and smad3 protein had a positive correlation with the level of alpha-SMA protein in cells (r = 0.901, P < than 0.01; r = 0.939, P < than 0.01).</p><p><b>CONCLUSIONS</b>Expression of smad3/alpha-SMA/beta-catenin is increased in the cultured HSC-T6 cells transfected by beta-catenin gene, especially when the transfected cells are stimulated by TGFbeta1. Our data suggest that beta-catenin could aggravate hepatic fibrosis induced by TGFbeta1.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Plásmidos
/
ARN Mensajero
/
Transfección
/
Línea Celular
/
Western Blotting
/
Actinas
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Proteínas Fluorescentes Verdes
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Hepatology
Año:
2009
Tipo del documento:
Artículo
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