Histomorphology and proteomics analysis of human fetus liver / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 46-49, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-250060
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the histomorphology and protein expression profiles of human fetal liver at different developmental stages.</p><p><b>METHODS</b>The protein expression patterns of human fetus livers at early and late stages were profiled by two-dimensional gel electrophoresis (2-DE) combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis. The differentially expressed proteins were identified by searching the protein databases with Mascot or ProFound softwares.</p><p><b>RESULTS</b>The obvious differences between early and late fetus liver were not only in morphology but also in protein expression profiles. Compared with protein expression pattern of hepatocellular carcinoma, 32 differential or similar protein spots were analyzed by MALDI-TOF MS, and 26 proteins, including proteins involved in cell proliferation, apoptosis and metabolism, were identified.</p><p><b>CONCLUSION</b>The data suggest that the hepatic cancer cells regain some characteristics of fetal hepatic cells during the process of carcinogenesis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas
/
Carcinoma Hepatocelular
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
/
Proteómica
/
Feto
/
Hígado
/
Neoplasias Hepáticas
/
Metabolismo
Tipo de estudio:
Estudio pronóstico
Límite:
Femenino
/
Humanos
/
Embarazo
Idioma:
Chino
Revista:
Chinese Journal of Hepatology
Año:
2009
Tipo del documento:
Artículo
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