Adenovirus mediated expression of interleukin 12 regulating hepatitis C virus E2 gene immunization-induced immune response / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 620-622, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-250130
ABSTRACT
<p><b>OBJECTIVE</b>To observe the regulating effect of hepatitis C virus (HCV) envelop (E) 2 gene immunization-induced immune responses by adenovirus mediated interleukin 12 (IL-12).</p><p><b>METHODS</b>HCV E2 protein was expressed and purified from NIH 3T3 and then used as an antigen to detect antibodies against HCV E2. With 51Cr release, SP2/0 expressing HCV E2 was used as target cell to detect specific cytotoxic T lymphocytes (CTL) response; adenovirus recombined IL-12 was propagated by 293 cell. HCV E2 recombinant and adenovirus recombined IL-12 were injected into the quadriceps femoris muscles and abdominal cavities of 6-8 weeks old BALB/C mice. Sera were collected at 2, 3, and 4 weeks and detected for antibodies for E2. Spleen cells isolated at 4 weeks were analyzed for specific CTL response.</p><p><b>RESULTS</b>It was found that expression of IL-12 at an undetectable level did enhance HCV E2 gene immunization-induced CTL activity and there was no effect on its hormonal immune response.</p><p><b>CONCLUSION</b>Using adenovirus to express interleukin 12 was helpful for regulation of HCV E2 gene immunization-induced immune response. Combined HCV E2 and IL-12 can render a strong anti-HCV CTL activity and may be of use in the development of HCV gene vaccine in the future.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Linfocitos T Citotóxicos
/
Adenoviridae
/
Proteínas del Envoltorio Viral
/
Interleucina-12
/
Alergia e Inmunología
/
Genética
Idioma:
Chino
Revista:
Chinese Journal of Hepatology
Año:
2004
Tipo del documento:
Artículo
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