Triton X-100 induces apoptosis in human hepatoma cell lines
Yonsei Medical Journal
; : 52-59, 1997.
Article
en En
| WPRIM
| ID: wpr-25046
Biblioteca responsable:
WPRO
ABSTRACT
The detergent Triton X-100 was used to establish a model for apoptosis in hepatoma cell lines. The electrophoresis of DNA extracted from 0.01% Triton X-100 treated hepatoma cell lines showed DNA ladder formation, a hallmark of apoptosis. The DNA fragmentation appeared within less than 60 min of the Triton X-100 treatment. Chromatin condensation and apoptotic bodies were observed by hematoxylin and eosin (H & E) stain, and fragmented nucleosome was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL) test. Apoptosis was semi-quantitated by measuring the lactate dehydrogenase (LDH) level for cytotoxity. It was found that apoptosis had been induced in more than 90% of the cells treated with Triton X-100 for 150 min. These data show that Triton X-100 efficiently induces the apoptotic cell death in hepatoma cell lines.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Células Tumorales Cultivadas
/
Apoptosis
/
Octoxinol
/
Carcinoma Hepatocelular
/
Detergentes
/
Fragmentación del ADN
/
Neoplasias Hepáticas
Límite:
Humans
Idioma:
En
Revista:
Yonsei Medical Journal
Año:
1997
Tipo del documento:
Article