Serum antibody response and Hantavirus RNA sequencing in patients with hemorrhagic fever renal syndrome in Yantai areas / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 124-128, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-250525
ABSTRACT
<p><b>OBJECTIVE</b>To understand antibody responses to and RNA sequences of Hantavirus in patients with hemorrhagic fever renal syndrome (HFRS) in Yantai areas and to demonstrate the type of the prevalent viruses caused HFRS.</p><p><b>METHODS</b>Serum specimens collected at acute and convalescent stages from 90 patients with HFRS and IgM and IgG antibodies against Hantavirus were detected with ELISA, and cross plaque reduction neutralizing tests were performed to detect neutralizing antibody. Viral RNA was extracted from the patients? sera by using Trizol method and nested PCR was utilized to amplify the specific segments of the viral cDNA and the products of the PCR were TA cloned and then the nucleotide sequences were determined.</p><p><b>RESULTS</b>The IgM antibody was positive in 82.2% (88/107) of the patients while the IgG antibody was positive in 85.7% (66/77) of the patients. Both the serologic and sequence analyses demonstrated that the epidemic of HFRS in Yantai areas was caused by mixed types of Hantavirus. The prevalent strains of Hantavirus had higher homology with the strains isolated in Korea than with those isolated previously in China.</p><p><b>CONCLUSIONS</b>The serologic and sequencing analyses indicated that the epidemic of HFRS in Yantai areas was caused by mixed types of Hantavirus dominated by type SEO.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Virología
/
Sangre
/
Reservorios de Enfermedades
/
ADN Viral
/
Inmunoglobulina G
/
Inmunoglobulina M
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Serotipificación
/
China
Límite:
Humanos
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2003
Tipo del documento:
Artículo
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