Determination of the binding rate of rat plasma protein with salvianolic acid B / 药学学报
Acta Pharmaceutica Sinica
; (12): 343-346, 2010.
Article
en Zh
| WPRIM
| ID: wpr-250581
Biblioteca responsable:
WPRO
ABSTRACT
This paper is aimed to report the development of a method for the determination of the binding rate of plasma protein with salvianolic acid B. In vitro, equilibrium dialysis method was used to imitate the binding process between salvianolic acid B and plasma protein, in vivo, ultrafiltration method was used and the binding rate with HPLC was determined. Plasma samples were treated with methanol to precipitate the protein, and the buffer solution was directly determined after filtering. The calibration curve of the buffer solution was linear in the range of 0.5-20 microg mL(-1). The calibration curve of the plasma was linear in the range of 2-200 microg mL(-1). The extract recovery was 68.6%-81.9%. RSDs of intra- and inter-day precisions were all less than 8.5%. The binding rates of plasma protein with salvianolic acid B in vitro was 75.2% and in vivo was 92.1%. This paper shows the high binding power of salvianolic acid B to plasma protein with high sensitivity, good reproduction, simple management and fulfilling the requirement.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Unión Proteica
/
Benzofuranos
/
Sangre
/
Medicamentos Herbarios Chinos
/
Proteínas Sanguíneas
/
Ultrafiltración
/
Química
/
Reproducibilidad de los Resultados
/
Cromatografía Líquida de Alta Presión
/
Sensibilidad y Especificidad
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Año:
2010
Tipo del documento:
Article