Evaluation by indirect immunofluorescent assay and enzyme linked immunosorbent assay of the dynamic changes of serum antibody responses against severe acute respiratory syndrome coronavirus / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 446-450, 2005.
Artículo
en Inglés
| WPRIM
| ID: wpr-250908
ABSTRACT
<p><b>BACKGROUND</b>Severe acute respiratory syndrome coronavirus (SARS-CoV) is a newly emerging virus that gives rise to SARS patients with high rates of infectivity and fatality. To study the humoral immune responses to SARS-CoV, the authors evaluated IgG and IgM specific antibodies in patients' sera.</p><p><b>METHODS</b>Two methods, enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescent assay (IFA), were used to detect specific serum IgG and IgM against SARS-CoV in 98 SARS patients and 250 controls consisting of patients with pneumonia, health-care professionals and healthy subjects. The serum antibody profiles were investigated at different times over one and a half years in 18 of the SARS patients.</p><p><b>RESULTS</b>The sensitivity and specificity of ELISA for detecting IgG against SARS-CoV were 100.0% and 97.2% and for IgM 89.8% and 97.6% respectively; the figures using IFA for IgG were 100.0% and 100.0% and for IgM 81.8% and 100.0% respectively. During the first seven days of the antibodies trace test, no IgG and IgM were detected, but on day 15, IgG response increased dramatically, reaching a peak on day 60, remaining high up to day 180 and decreasing gradually until day 540. On day 15, IgM was detected, rapidly reached a peak, then declined gradually until day 180 when IgM was undetectable.</p><p><b>CONCLUSION</b>The detection of antibodies against SARS virus is helpful in the clinical diagnosis of SARS.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sangre
/
Inmunoglobulina G
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Inmunoglobulina M
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Ensayo de Inmunoadsorción Enzimática
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Linfocitos T Citotóxicos
/
Técnica del Anticuerpo Fluorescente Indirecta
/
Síndrome Respiratorio Agudo Grave
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Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo
/
Alergia e Inmunología
/
Anticuerpos Antivirales
Límite:
Adulto
/
Anciano
/
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Chinese Medical Journal
Año:
2005
Tipo del documento:
Artículo
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