Effects of sodium magnesium fructose diphosphate on free calcium concentration and nitric oxide synthase activity of ischemic synaptosome / 药学学报
Acta Pharmaceutica Sinica
;
(12): 325-327, 2003.
Artículo
en Inglés
| WPRIM
| ID: wpr-251093
ABSTRACT
<p><b>AIM</b>To study the effects of sodium magnesium fructose diphosphate (SMFD) on free calcium concentration and nitric oxide synthase activity of ischemic synaptosome, so as to explore the protective mechanisms of SMFD on cerebral ischemia.</p><p><b>METHODS</b>The synaptosomes from normal rat brain were prepared by phase partition and cultured with oxygen-glucose deprivation to establish ischemic synaptosome model. The intrasynaptosomal free calcium concentration and nitric oxide synthase activity were detected separately after the synaptosomes were co-incubated with SMFD (1.3 mmol.L-1) or fructose-1, 6-diphosphate (FDP, 4.0 mmol.L-1) for 60 min.</p><p><b>RESULTS</b>SMFD decreased the free calcium concentration and reduced the activity of nitric oxide synthase (NOS) of ischemic synaptosomes. Its effects were more powerful than those of FDP.</p><p><b>CONCLUSION</b>SMFD may protect neurons from ischemic injury by preventing intracellular Ca2+ overload and inhibiting the activity of nitric oxide synthase.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Sodio
/
Sinaptosomas
/
Isquemia Encefálica
/
Quelantes
/
Química
/
Calcio
/
Ratas Wistar
/
Óxido Nítrico Sintasa
/
Fructosadifosfatos
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Acta Pharmaceutica Sinica
Año:
2003
Tipo del documento:
Artículo
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